The directors of Quarmby Construction said the company is unaware of any bid-rigging or cover pricing relating to past or current tender processes in which it has participated.
But Quarmby, which is headquartered in Ilkley, said it will co-operate with the Office of Fair Trading investigation.
The OFT said more than 40 companies had already admitted price-fixing in the inquiry so far, which first started after an initial complaint in the East Midlands.
Another 37 firms out of the 112 have applied for leniency, according to the OFT.
The inquiry spans 240 alleged cases where firms have colluded to inflate prices during a tender process, covering both the private and public sector, including building contracts for schools, universities and hospitals.