High Speed 2 will recruit more than 100 specialists to work on the design, engineering and construction of the £42.6bn project.
The jobs, which will be based in Birmingham, will include opportunities in civil, rail and site engineering, project management, corporate procurement, contract and commercial management.
There will also be roles in health and safety, accountancy, human resources and IT, as well as positions for legal specialists.
The announcement comes ahead of a two-day careers event being held by HS2 Ltd in Birmingham on the 17 and 18 September.
The event will give interested parties the chance to discuss the opportunities available and talk with staff currently working on the project.
The news comes as contractors ready themselves for the start of the procurement process for HS2’s main civils contracts, which is expected to begin in October.
The work up for grabs will include seven civils packages across the HS2 route, each worth between £700m and £1.1bn.
Commenting on the new jobs, HS2 Ltd chief executive Simon Kirby said: “The arrival of HS2 in Birmingham will play a vital role in boosting jobs, skills, economic growth and regeneration across the city and the West Midlands.
“HS2 will demand a wide range of skills across a broad range of areas, as the project develops from planning and design through to construction.
“We want to attract the best talent from across the country to work on HS2, Britain’s largest infrastructure project, and to encourage greater diversity among those entering a career in engineering, construction and the built environment.”
Birmingham City Council leader Sir Albert Bore said: “With some of the UK’s leading universities located in Birmingham and one of the youngest populations in Europe, this region has a vast and ambitious talent pool with expertise ranging from engineering and manufacturing to professional and financial services.
“This careers fair is one of the first opportunities for that talent pool to get involved with this country’s biggest infrastructure project in years.”
The event will be held at The Studio, 7 Cannon Street, Birmingham, B2 5EP on Thursday 17 September 10am-7pm and Friday 18 September 9am-5pm.
Meanwhile, HS2 Ltd has called on Northern Irish companies to bid for the £10bn-worth of contracts up for grabs.
In an appeal to firms in Northern Ireland, HS2 Ltd commercial director Beth West said: “To deliver a project of HS2’s scale and complexity requires the right companies with the right skills.
“I’m extremely pleased that so many local firms have already shown an interest and I’d like to encourage all Northern Ireland businesses large or small to get involved with HS2.”
Junior transport minister Robert Goodwill said: “HS2 provides an excellent opportunity for businesses across the UK, including Northern Ireland, to be part of one of Europe’s largest infrastructure projects.
“As a one nation government, we want the whole country to benefit from HS2 – and well in advance of trains actually running on the line.”